The chromosome-scale reference genome of mirid bugs (Adelphocoris suturalis) genome provides insights into omnivory, insecticide resistance, and survival adaptation
Zhongping Xu,
Guanying Wang,
Jing Luo,
Mingju Zhu,
Lisong Hu,
Sijia Liang,
Bo Li,
Xingxing Huang,
Ying Wang,
Guangyu Zhang,
Can Zhang,
Yi Zhou,
Daojun Yuan,
Taiyu Chen,
Lizhen Chen,
Weihua Ma,
Wei Gao,
Keith Lindsey,
Xianlong Zhang,
Fang Ding,
Shuangxia Jin
Affiliations
Zhongping Xu
National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University
Guanying Wang
National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University
Jing Luo
State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University
Mingju Zhu
National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University
Lisong Hu
Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences
Sijia Liang
Academy of Industry Innovation and Development, Huanghuai University
Bo Li
Xinjiang Key Laboratory of Crop Biotechnology, Institute of Nuclear and Biological Technology, Xinjiang Academy of Agricultural Sciences
Xingxing Huang
Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, Huazhong Agricultural University
Ying Wang
National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University
Guangyu Zhang
National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University
Can Zhang
National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University
Yi Zhou
National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University
Daojun Yuan
National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University
Taiyu Chen
National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University
Lizhen Chen
Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, Huazhong Agricultural University
Weihua Ma
National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University
Wei Gao
State Key Laboratory of Cotton Biology, Henan Key Laboratory of Plant Stress Biology, School of Life Science, Henan University
Keith Lindsey
Department of Biosciences, Durham University
Xianlong Zhang
National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University
Fang Ding
Hubei Key Laboratory of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University
Shuangxia Jin
National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University
Abstract Background Adelphocoris suturalis (Hemiptera: Miridae) is a notorious agricultural pest, which causes serious economic losses to a diverse range of agricultural crops around the world. The poor understanding of its genomic characteristics has seriously hindered the establishment of sustainable and environment-friendly agricultural pest management through biotechnology and biological insecticides. Results Here, we report a chromosome-level assembled genome of A. suturalis by integrating Illumina short reads, PacBio, 10x Chromium, and Hi-C mapping technologies. The resulting 1.29 Gb assembly contains twelve chromosomal pseudomolecules with an N50 of 1.4 and 120.6 Mb for the contigs and scaffolds, respectively, and carries 20,010 protein-coding genes. The considerable size of the A. suturalis genome is predominantly attributed to a high amount of retrotransposons, especially long interspersed nuclear elements (LINEs). Transcriptomic and phylogenetic analyses suggest that A. suturalis-specific candidate effectors, and expansion and expression of gene families associated with omnivory, insecticide resistance and reproductive characteristics, such as digestion, detoxification, chemosensory receptors and long-distance migration likely contribute to its strong environmental adaptability and ability to damage crops. Additionally, 19 highly credible effector candidates were identified and transiently overexpressed in Nicotiana benthamiana for functional assays and potential targeting for insect resistance genetic engineering. Conclusions The high-quality genome of A. suturalis provides an important genomic landscape for further investigations into the mechanisms of omnivory, insecticide resistance and survival adaptation, and for the development of integrated management strategies.
