Surfactant Lipidomics of Alveolar Lavage Fluid in Mice Based on Ultra-High-Performance Liquid Chromatography Coupled to Hybrid Quadrupole-Exactive Orbitrap Mass Spectrometry
Rui Yang,
Ying Zhang,
Wenjuan Qian,
Linxiu Peng,
Lili Lin,
Jia Xu,
Tong Xie,
Jianjian Ji,
Xiuqin Zhan,
Jinjun Shan
Affiliations
Rui Yang
Jiangsu Key Laboratory of Pediatric Respiratory Disease, School of Medicine and Life Sciences, Nanjing University of Chinese Medicine, Nanjing 210023, China
Ying Zhang
Genome Center of UC Davis, NIH West Coast Metabolomics Center, Davis, CA 95616, USA
Wenjuan Qian
Jiangsu Key Laboratory of Pediatric Respiratory Disease, School of Medicine and Life Sciences, Nanjing University of Chinese Medicine, Nanjing 210023, China
Linxiu Peng
Medical Metabolomics Center, Nanjing University of Chinese Medicine, Nanjing 210023, China
Lili Lin
Medical Metabolomics Center, Nanjing University of Chinese Medicine, Nanjing 210023, China
Jia Xu
Medical Metabolomics Center, Nanjing University of Chinese Medicine, Nanjing 210023, China
Tong Xie
Medical Metabolomics Center, Nanjing University of Chinese Medicine, Nanjing 210023, China
Jianjian Ji
Institute of Pediatrics, Nanjing University of Chinese Medicine, Nanjing 210023, China
Xiuqin Zhan
Jiangsu Key Laboratory of Pediatric Respiratory Disease, School of Medicine and Life Sciences, Nanjing University of Chinese Medicine, Nanjing 210023, China
Jinjun Shan
Jiangsu Key Laboratory of Pediatric Respiratory Disease, School of Medicine and Life Sciences, Nanjing University of Chinese Medicine, Nanjing 210023, China
Surfactant lipid metabolism is closely related to pulmonary diseases. Lipid metabolism disorder can cause lung diseases, vice versa. With this rationale, a useful method was established in this study to determine the lipidome in bronchoalveolar lavage fluid (BALF) of mice. The lipid components in BALF were extracted by liquid−liquid extraction (methanol and methyl tert-butyl ether, and water). Ultra-high-performance liquid chromatography coupled to hybrid Quadrupole-Exactive Orbitrap mass spectrometry was used to analyze the extracted samples, which showed a broad scanning range of 215−1800 m/z. With MS-DIAL software and built-in LipidBlast database, we identified 38 lipids in positive, and 31 lipids in negative, ion mode, including lysophosphatidylcholine (lysoPC), phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), etc. Then, the changes of lipids in BALF of mice with acute lung injury (ALI) induced by lipopolysaccharide (LPS) was investigated, which may contribute to further exploration of the pathogenesis of ALI.
