Intestinal Lipase Characterization in Common Snook (<em>Centropomus undecimalis</em>) Juveniles
Bartolo Concha-Frías,
Martha Gabriela Gaxiola-Cortes,
Fanny Janet De la Cruz-Alvarado,
Luis Daniel Jimenez Martinez,
Emyr Saul Peña-Marin,
Marcia Angélica Oliva-Arriagada,
Joe Luis Arias-Moscoso,
Carlos Alfonso Alvarez-González
Affiliations
Bartolo Concha-Frías
Laboratorio de Biología Molecular, DAMJ-UJAT, Carretera Estatal Libre Villahermosa-Comalcalco Km. 27+000 s/n Ranchería Ribera Alta, Jalpa de Méndez 86205, Mexico
Martha Gabriela Gaxiola-Cortes
Unidad Multidisciplinaria de Docencia e Investigación, Facultad de Ciencias, UNAM, Puerto de Abrigo s/n, Sisal 97356, Mexico
Fanny Janet De la Cruz-Alvarado
Laboratorio de Fisiología en Recursos Acuáticos, DACBIOL-Universidad Juárez Autónoma de Tabasco, Carretera Villahermosa Cárdenas Km 0.5, Villahermosa 86139, Mexico
Luis Daniel Jimenez Martinez
Laboratorio de Biología Molecular, DAMJ-UJAT, Carretera Estatal Libre Villahermosa-Comalcalco Km. 27+000 s/n Ranchería Ribera Alta, Jalpa de Méndez 86205, Mexico
Emyr Saul Peña-Marin
Laboratorio de Fisiología en Recursos Acuáticos, DACBIOL-Universidad Juárez Autónoma de Tabasco, Carretera Villahermosa Cárdenas Km 0.5, Villahermosa 86139, Mexico
Marcia Angélica Oliva-Arriagada
Laboratorio de Peces, Departamento de Acuicultura, Facultad de Ciencias del Mar, Universidad Católica de Chile, Campus Guayacán Larrondo 1281, Coquimbo 1780000, Chile
Joe Luis Arias-Moscoso
Department of Engineering, Technological National of Mexico, Technological Institute of the Yaqui Valley, Bacum 85276, Mexico
Carlos Alfonso Alvarez-González
Laboratorio de Fisiología en Recursos Acuáticos, DACBIOL-Universidad Juárez Autónoma de Tabasco, Carretera Villahermosa Cárdenas Km 0.5, Villahermosa 86139, Mexico
The common snook (Centropomus undecimalis) is a euryhaline fish with high commercial demand in the Mexican southeast, Caribbean, and South America. However, some aspects of its digestive physiology are still unknown, particularly in relation to lipid hydrolysis. Therefore, the characterization of the digestive lipase of this species was carried out. Our results show that the digestive lipase’s optimal temperature is 35 °C, being stable between 25 and 35 °C, and shows maximum activity at pH 9, with stability between pH 5 and 8. Different degrees of inhibition were presented by Orlistat (61.4%), Ebelactone A (90.36%), Ebelactone B (75.9%), SDS 1% (80.7%), SDS 0.1% (73.5%), and SDS at 0.01% (34.9%). Orlistat and Ebelactone A and B completely inhibited the lipase band in the zymogram, but not SDS addition. Lipase showed a molecular weight of 43.8 kDa. The high lipase activities in the digestive tract indicate the importance of lipids in the diet of C. undecimalis.
