Differential Expression of LncRNA in Bladder Cancer Development
Lorenzo Spirito,
Rufina Maturi,
Sara Carmela Credendino,
Celeste Manfredi,
Davide Arcaniolo,
Marco De Martino,
Francesco Esposito,
Luigi Napolitano,
Francesco Di Bello,
Alfredo Fusco,
Pierlorenzo Pallante,
Marco De Sio,
Gabriella De Vita
Affiliations
Lorenzo Spirito
Urology Unit, Department of Woman Child and General and Specialized Surgery, University of Campania “Luigi Vanvitelli”, Via De Crecchio 7, 80138 Naples, Italy
Rufina Maturi
Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Via Pansini 5, 80131 Naples, Italy
Sara Carmela Credendino
Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Via Pansini 5, 80131 Naples, Italy
Celeste Manfredi
Urology Unit, Department of Woman Child and General and Specialized Surgery, University of Campania “Luigi Vanvitelli”, Via De Crecchio 7, 80138 Naples, Italy
Davide Arcaniolo
Urology Unit, Department of Woman Child and General and Specialized Surgery, University of Campania “Luigi Vanvitelli”, Via De Crecchio 7, 80138 Naples, Italy
Marco De Martino
Institute of Experimental Endocrinology and Oncology “G. Salvatore”, National Research Council (CNR), Via Pansini, 5, 80131 Naples, Italy
Francesco Esposito
Institute of Experimental Endocrinology and Oncology “G. Salvatore”, National Research Council (CNR), Via Pansini, 5, 80131 Naples, Italy
Luigi Napolitano
Urology Unit, Department of Neurosciences, Reproductive Sciences, and Odontostomatology, University of Naples Federico II, Via Pansini 5, 80131 Naples, Italy
Francesco Di Bello
Urology Unit, Department of Neurosciences, Reproductive Sciences, and Odontostomatology, University of Naples Federico II, Via Pansini 5, 80131 Naples, Italy
Alfredo Fusco
Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Via Pansini 5, 80131 Naples, Italy
Pierlorenzo Pallante
Institute of Experimental Endocrinology and Oncology “G. Salvatore”, National Research Council (CNR), Via Pansini, 5, 80131 Naples, Italy
Marco De Sio
Urology Unit, Department of Woman Child and General and Specialized Surgery, University of Campania “Luigi Vanvitelli”, Via De Crecchio 7, 80138 Naples, Italy
Gabriella De Vita
Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Via Pansini 5, 80131 Naples, Italy
Bladder cancer (BC) is the tenth most common cancer, with urothelial carcinoma representing about 90% of all BC, including neoplasms and carcinomas of different grades of malignancy. Urinary cytology has a significant role in BC screening and surveillance, although it has a low detection rate and high dependence on the pathologist’s experience. The currently available biomarkers are not implemented into routine clinical practice due to high costs or low sensitivity. In recent years, the role of lncRNAs in BC has emerged, even though it is still poorly explored. We have previously shown that the lncRNAs Metallophosphoesterase Domain-Containing 2 Antisense RNA 1 (MPPED2-AS1), Rhabdomyosarcoma-2 Associated Transcript (RMST), Kelch-like protein 14 antisense (Klhl14AS) and Prader Willi/Angelman region RNA 5 (PAR5) are involved in the progression of different types of cancers. Here, we investigated the expression of these molecules in BC, first by interrogating the GEPIA database and observing a different distribution of expression levels between normal and cancer specimens. We then measured them in a cohort of neoplastic bladder lesions, either benign or malignant, from patients with suspicion of BC undergoing transurethral resection of bladder tumor (TURBT). The total RNA from biopsies was analyzed using qRT-PCR for the expression of the four lncRNA genes, showing differential expression of the investigated lncRNAs between normal tissue, benign lesions and cancers. In conclusion, the data reported here highlight the involvement of novel lncRNAs in BC development, whose altered expression could potentially affect the regulatory circuits in which these molecules are involved. Our study paves the way for testing lncRNA genes as markers for BC diagnosis and/or follow-up.
