Mungbean Yellow Mosaic India Virus (MYMIV)-infection, Small RNA Library Construction and Deep Sequencing for MicroRNA Identification in Vigna mungo

Anirban Kundu; Sujay Paul; Amita Pal; Genotypic Technology

doi:10.21769/BioProtoc.1961

Bio-Protocol (Oct 2016)

Mungbean Yellow Mosaic India Virus (MYMIV)-infection, Small RNA Library Construction and Deep Sequencing for MicroRNA Identification in Vigna mungo

Anirban Kundu,
Sujay Paul,
Amita Pal,
Genotypic Technology

Affiliations

Anirban Kundu: Division of Plant Biology, Bose Institute, Kolkata, West Bengal, India
Sujay Paul: Division of Plant Biology, Bose Institute, Kolkata, West Bengal, India
Amita Pal: Division of Plant Biology, Bose Institute, Kolkata, West Bengal, India
Genotypic Technology: Genotypic Technology Private Limited, #2/13, Balaji Complex, Bangalore, India

DOI: https://doi.org/10.21769/BioProtoc.1961
Journal volume & issue: Vol. 6, no. 20

Abstract

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This protocol describes small RNA library preparation from Vigna mungo total RNA followed by deep sequencing and analysis for microRNA identification.

Published in Bio-Protocol

ISSN: 2331-8325 (Online)
Publisher: Bio-protocol LLC
Country of publisher: United States
LCC subjects: Science: Biology (General)
Website: https://bio-protocol.org

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