Iranian Journal of Public Health (Oct 2016)

Molecular Characterization of Animal Fasciola spp. Isolates from Kermanshah, Western Iran

  • Arezoo BOZORGOMID,
  • Naser NAZARI,
  • Hamzeh RAHIMI,
  • Eshrat BEIGOM KIA,
  • Homa HAJJARAN,
  • Mehdi MOHEBALI,
  • Mojgan ARYAEIPOUR,
  • Peyman HEIDARIAN,
  • Mohammad Saeid EZATI,
  • Mohamad Bagher ROKNI

Journal volume & issue
Vol. 45, no. 10
pp. 1315 – 1321

Abstract

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Background: We evaluated the genetic diversity of samples identified morphologically as Fasciola spp. from sheep, cattle and goat from Kermanshah Province, western Iran using PCR-RFLP method.Methods: We used PCR-RFLP analysis of ribosomal ITS1 fragment using RsaI restriction enzyme to investigate the genetic characteristics of Fasciola species obtained from different hosts (16 sheep, 28 cattle, 4 goats). The species of Fasciola were confirmed by sequencing the 700 bp region of ribosomal ITS1 gene.Results: In Kermanshah, F. hepatica was present in 96% of the samples, F. gigantica was found only in two cattle sample. No hybrid forms were detected in the present study. Conclusion: Our results contribute to clarify the dark spots of Fasciola genotyping in different parts of Iran. Keywords: Fasciola hepatica, Fasciola gigantica, Genotype, PCR, Iran

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