Variability of leaf pubescence characteristics in transgenic tobacco lines with partial proline dehydrogenase gene suppression

Abstract

Proline, an amino acid, plays an important role in plants, and it is involved in stress resistance and development. Earlier, to study the proline role in maintaining stress resistance in plants, we obtained genetically modified transgenic lines of tobacco (Nicotiana tabacum L.) with reduced activity of proline dehydrogenase (PDH, the proline degradation gene) and increased content of proline. Transgenic tobacco plants demonstrated greater resistance to high concentrations of NaCl, drought, low temperatures, and heavy metals vs. control plants. The visual assessment showed that the leaf pubescence in transgenic plants varied noticeably. Here we apply automated analysis of the tobacco leaf folds to estimate quantitative characteristics of pubescence in genetically modified tobacco plants and the control SR1 line under non-stress conditions. Our results showed differences in the number of trichomes and their length between transgenic and control plants. The trichome number significantly increased in transgenic plants (from 1.5 to 3 times). The largest differences in the trichome numbers were observed for trichomes with lengths from 0 to 380 µm. When assessing the trichome length, the opposite was observed. In all three transgenic lines, the trichome length was significantly lower than that of the control SR1 line. The data obtained indicate the effect of proline as an important metabolome component affecting the plant phenotype. Our results demonstrate perspectives of tobacco transgenic lines as promising genetic models for studying the proline role in plant morphogenesis.

Keywords

• image analysis
• leaf trichomes
• nicotiana tabacum
• proline
• proline dehydrogenase
• transgenic lines