Effect of aqueous extract of Morus nigra on skin wound healing in type 1 diabetic rats

Abstract

Introduction: Delay in wound healing is one of several complications of type 1 diabetes. It has been reported that the use of various herbal extracts such as Morus nigra increase the  wound healing.  Since,there are no studies on investigation of the effects of Morus nigra on wound healing in diabetics so this study was conducted to determine wound healing effects of Morus nigra in diabetic rats. Methods: In this experimental study, the fresh fruits were blended and pressed. Then, the extract was filtered and then was passed through the filter paper then it was dried in shade. Diabetes was induced by injection of single dose of streptozotocin (60 mg/kg). The circular wound (diameter 8 cm) was created on the back of each rat. The wounds were topically treated with 10 or 20 percent of concentration of the extract. Wounds in control group were treated with saline and normal group received no treatment.  Wound areas were monitored on days 1, 6 and 11; the repaired tissues were removed for histological evaluations. Result: Daily and local applications of the extract displayed significant wound healing activity through the reducing wound area and acceleration of wound contraction. Histological findings showed that various indices of tissue repairment such as  integrated epithelial,  typical granulated and regular collagen layers were mor obvious in  the treated wounds. Conclusion: Topical application of the Morus nigra extract can accelrate wound contraction and improve the histological indices inclue formation of epithelial and granulated layers and increase collagen density in the repaired tissue.

Keywords

• Wound Healing
• Diabetes
• Rat
• Morus Nigra
• Epithelial
• Collagen