Discovery of traditional Chinese medicines against porcine reproductive and respiratory syndrome virus

Abstract

Porcine reproductive and respiratory syndrome (PRRS) is a highly contagious disease caused by the porcine reproductive and respiratory syndrome virus (PRRSV). It is characterized by reproductive disorders in pregnant sows and respiratory symptoms in pigs of all ages. Although antiviral drugs, vaccines, and improved management have played a role in the prevention and control of the PRRS, this disease is still prevalent in pig farms. Hence, the prevention, control, and purification of the PRRS have been the main focus. As we all know, traditional Chinese medicines (TCMs) have played a significant role in the history of ancient medicine. Therefore, exploring effective anti-PRRSV TCMs is an essential way to solve the prevention and treatment of PRRSV. In this study, the active sites of the fifth scavenger receptor cysteine-rich domain of the CD163 receptor was first studied by computer-aided drug design technology. Second, virtual screening of TCMs monomers that may bind to active site was conducted. Third, the crude extracts of TCMs rich in the matching monomer were obtained. Finally, the anti-PRRSV effect of crude extracts of TCMs were verified in vitro. To sum up, it was found that Plantago ovata, Rhus chinensis, and Persicaria orientalis had anti-PRRSV effects in vitro, which laid a theoretical foundation for the research and development of anti-PRRSV TCMs. In addition, this exploration strategy could provide a research template for the discovery of TCMs for human viral diseases.

Keywords

• PRRSV
• CD163
• Virtual screening
• Molecular dynamics
• Traditional Chinese medicines