The Fusion Gene <i>BPI-LY</i>, Encoding Human Bactericidal/Permeability-Increasing Protein Core Fragments and Lysozyme, Enhanced the Resistance of Transgenic Tomato Plants to Bacterial Wilt
Lei Ni,
Yue Zhang,
Yafei Qin,
Mei Wang,
Daodao Tang,
Liantian Chen,
Xing Ding,
Yilin Zheng,
Yu Pan,
Jinhua Li,
Xingguo Zhang
Affiliations
Lei Ni
College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China
Yue Zhang
College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China
Yafei Qin
College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China
Mei Wang
College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China
Daodao Tang
College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China
Liantian Chen
College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China
Xing Ding
College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China
Yilin Zheng
College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China
Yu Pan
College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China
Jinhua Li
College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China
Xingguo Zhang
College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China
Tomato bacterial wilt, caused by Ralstonia solanacearum (G−), is one of the most devastating plant diseases. Developing effective resistance against this pathogen remains a major challenge in plant disease management. In this study, we constructed a fusion gene BPI-LY by combining the gene encoding the lipophilic functional domains of human bactericidal/permeability-increasing protein (BPI) with the gene of human lysozyme (LY). The recombinant gene BPI-LY was heterologously expressed in yeast and tomato. Preliminary in vitro assays in yeast demonstrated that BPI enhances LY’s antibacterial activity against G− bacteria. Furthermore, overexpression of BPI-LY in tomato delayed onset of the disease in the transgenic lines and lowered the degree of tissue damage and the number of bacteria present in the stems relative to those in the wild-type plant. Additionally, the expression levels of the SlSOD, SlPOD, SlPAL, SlPR5, SlPR10, and SlPR-NP24 genes were indirectly upregulated in the transgenic plants following R. solanacearum inoculation. Collectively, these findings demonstrate that BPI-LY enhances the resistance of transgenic tomato against bacterial wilt caused by R. solanacearum.
