Serum Metabolomic Profile in Hypoxia-Induced Pulmonary Hypertension Mice after C75 Treatment

Shun Chen; Shujia Lin; Wei Liu; Qiuping Lin; Yi Yang; Qingzhu Qiu; Yanfang Zong; Tingting Xiao; Cuilan Hou; Lijian Xie

doi:10.31083/j.fbl2810251

Frontiers in Bioscience-Landmark (Oct 2023)

Serum Metabolomic Profile in Hypoxia-Induced Pulmonary Hypertension Mice after C75 Treatment

Shun Chen,
Shujia Lin,
Wei Liu,
Qiuping Lin,
Yi Yang,
Qingzhu Qiu,
Yanfang Zong,
Tingting Xiao,
Cuilan Hou,
Lijian Xie

Affiliations

Shun Chen: Department of Cardiology, Shanghai Children’s Hospital, School of medicine, Shanghai Jiao Tong University, 200025 Shanghai, China
Shujia Lin: Department of Cardiology, Shanghai Children’s Hospital, School of medicine, Shanghai Jiao Tong University, 200025 Shanghai, China
Wei Liu: Department of Cardiology, Shanghai Children’s Hospital, School of medicine, Shanghai Jiao Tong University, 200025 Shanghai, China
Qiuping Lin: Department of Cardiology, Shanghai Children’s Hospital, School of medicine, Shanghai Jiao Tong University, 200025 Shanghai, China
Yi Yang: Department of Cardiology, Shanghai Children’s Hospital, School of medicine, Shanghai Jiao Tong University, 200025 Shanghai, China
Qingzhu Qiu: Department of Cardiology, Shanghai Children’s Hospital, School of medicine, Shanghai Jiao Tong University, 200025 Shanghai, China
Yanfang Zong: Department of Cardiology, Shanghai Children’s Hospital, School of medicine, Shanghai Jiao Tong University, 200025 Shanghai, China
Tingting Xiao: Department of Cardiology, Shanghai Children’s Hospital, School of medicine, Shanghai Jiao Tong University, 200025 Shanghai, China
Cuilan Hou: Department of Cardiology, Shanghai Children’s Hospital, School of medicine, Shanghai Jiao Tong University, 200025 Shanghai, China
Lijian Xie: Department of Cardiology, Shanghai Children’s Hospital, School of medicine, Shanghai Jiao Tong University, 200025 Shanghai, China

DOI: https://doi.org/10.31083/j.fbl2810251
Journal volume & issue: Vol. 28, no. 10
p. 251

Abstract

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Background: Inhibition of fatty acid synthase (FAS) plays a crucial protective role in pulmonary hypertension (PH). Our aim was to identify novel metabolites in mice with hypoxia-induced PH after treatment with C75 (FAS inhibitor) and to confirm the presence of these metabolites in paediatric patients with PH. Methods: The PH mouse model was built by chronic hypoxia and ovalbumin (OVA) assistance. Untargeted metabolomics was used to analyse mouse serum. Six children with PH and six relative controls (patients without lung and heart disease) were selected in Shanghai Children’s Hospital and they all performed blood tandem mass spectrometry during hospitalization. Results: First, a total of 29 differential metabolites, including lipid metabolites, polyamine, and glutamine were identified as differential metabolites in the hypoxia group compared with the control group. After C75 treatment, symptoms were partially relieved in the PH mouse, and 15 differential metabolites, including lipid metabolites, polyamine, and glutamine were identified in the hypoxia + C75 group compared with the hypoxia group. These differential metabolites were enriched in arginine and glycerolipid metabolism through metabolite set enrichment analyses and were involved in excessive cell proliferation, which was a characteristic of PH. Second, glutamine and caproyl carnitine levels were increased in paediatric patients with PH. Conclusions: FAS may be a potential PH therapeutic target. Lipid metabolites, polyamine, and glutamine, are closely related to PH. Putrescine and glutamine might be biomarkers for PH.

Published in Frontiers in Bioscience-Landmark

ISSN: 2768-6701 (Print); 2768-6698 (Online)
Publisher: IMR Press
Country of publisher: Singapore
LCC subjects: Science: Chemistry: Organic chemistry: Biochemistry; Science: Biology (General)
Website: https://www.imrpress.com/journal/FBL

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