A comprehensive thermodynamic model for RNA binding by the Saccharomyces cerevisiae Pumilio protein PUF4

Christoph Sadée; Lauren D. Hagler; Winston R. Becker; Inga Jarmoskaite; Pavanapuresan P. Vaidyanathan; Sarah K. Denny; William J. Greenleaf; Daniel Herschlag

doi:10.1038/s41467-022-31968-z

Nature Communications (Aug 2022)

A comprehensive thermodynamic model for RNA binding by the Saccharomyces cerevisiae Pumilio protein PUF4

Christoph Sadée,
Lauren D. Hagler,
Winston R. Becker,
Inga Jarmoskaite,
Pavanapuresan P. Vaidyanathan,
Sarah K. Denny,
William J. Greenleaf,
Daniel Herschlag

Affiliations

Christoph Sadée: Department of Biochemistry, Stanford University School of Medicine
Lauren D. Hagler: Department of Biochemistry, Stanford University School of Medicine
Winston R. Becker: Biophysics Program, Stanford University School of Medicine
Inga Jarmoskaite: Department of Biochemistry, Stanford University School of Medicine
Pavanapuresan P. Vaidyanathan: Department of Biochemistry, Stanford University School of Medicine
Sarah K. Denny: Biophysics Program, Stanford University School of Medicine
William J. Greenleaf: Department of Genetics, Stanford University School of Medicine
Daniel Herschlag: Department of Biochemistry, Stanford University School of Medicine

DOI: https://doi.org/10.1038/s41467-022-31968-z
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 15

Abstract

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Traditional genomic methods identify RNA-binding proteins (RBPs) and the genes they regulate, but do not provide predictive models. The authors used an emerging technology to obtain a complete thermodynamic model for RNA binding to the PUF4 RBP.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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