Frontiers in Physiology (May 2020)
Taurine Alleviates Intestinal Injury by Mediating Tight Junction Barriers in Diquat-Challenged Piglet Models
- Chaoyue Wen,
- Chaoyue Wen,
- Chaoyue Wen,
- Chaoyue Wen,
- Chaoyue Wen,
- Chaoyue Wen,
- Chaoyue Wen,
- Chaoyue Wen,
- Qiuping Guo,
- Qiuping Guo,
- Qiuping Guo,
- Qiuping Guo,
- Qiuping Guo,
- Qiuping Guo,
- Wenlong Wang,
- Wenlong Wang,
- Wenlong Wang,
- Wenlong Wang,
- Wenlong Wang,
- Wenlong Wang,
- Wenlong Wang,
- Wenlong Wang,
- Yehui Duan,
- Yehui Duan,
- Yehui Duan,
- Yehui Duan,
- Yehui Duan,
- Lingyu Zhang,
- Lingyu Zhang,
- Lingyu Zhang,
- Lingyu Zhang,
- Lingyu Zhang,
- Lingyu Zhang,
- Jianzhong Li,
- Jianzhong Li,
- Jianzhong Li,
- Shanping He,
- Shanping He,
- Shanping He,
- Wen Chen,
- Wen Chen,
- Wen Chen,
- Wen Chen,
- Wen Chen,
- Fengna Li,
- Fengna Li,
- Fengna Li,
- Fengna Li,
- Fengna Li
Affiliations
- Chaoyue Wen
- Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Changsha, China
- Chaoyue Wen
- Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, China
- Chaoyue Wen
- Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Changsha, China
- Chaoyue Wen
- National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Changsha, China
- Chaoyue Wen
- Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, China
- Chaoyue Wen
- Laboratory of Animal Nutrition and Human Health, College of Life Science, Hunan Normal University, Changsha, China
- Chaoyue Wen
- Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, College of Life Science, Hunan Normal University, Changsha, China
- Chaoyue Wen
- Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, College of Life Science, Hunan Normal University, Changsha, China
- Qiuping Guo
- Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Changsha, China
- Qiuping Guo
- Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, China
- Qiuping Guo
- Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Changsha, China
- Qiuping Guo
- National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Changsha, China
- Qiuping Guo
- Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, China
- Qiuping Guo
- University of Chinese Academy of Sciences, Beijing, China
- Wenlong Wang
- Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Changsha, China
- Wenlong Wang
- Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, China
- Wenlong Wang
- Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Changsha, China
- Wenlong Wang
- National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Changsha, China
- Wenlong Wang
- Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, China
- Wenlong Wang
- Laboratory of Animal Nutrition and Human Health, College of Life Science, Hunan Normal University, Changsha, China
- Wenlong Wang
- Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, College of Life Science, Hunan Normal University, Changsha, China
- Wenlong Wang
- Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, College of Life Science, Hunan Normal University, Changsha, China
- Yehui Duan
- Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Changsha, China
- Yehui Duan
- Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, China
- Yehui Duan
- Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Changsha, China
- Yehui Duan
- National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Changsha, China
- Yehui Duan
- Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, China
- Lingyu Zhang
- Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Changsha, China
- Lingyu Zhang
- Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, China
- Lingyu Zhang
- Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Changsha, China
- Lingyu Zhang
- National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Changsha, China
- Lingyu Zhang
- Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, China
- Lingyu Zhang
- University of Chinese Academy of Sciences, Beijing, China
- Jianzhong Li
- Laboratory of Animal Nutrition and Human Health, College of Life Science, Hunan Normal University, Changsha, China
- Jianzhong Li
- Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, College of Life Science, Hunan Normal University, Changsha, China
- Jianzhong Li
- Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, College of Life Science, Hunan Normal University, Changsha, China
- Shanping He
- Laboratory of Animal Nutrition and Human Health, College of Life Science, Hunan Normal University, Changsha, China
- Shanping He
- Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, College of Life Science, Hunan Normal University, Changsha, China
- Shanping He
- Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, College of Life Science, Hunan Normal University, Changsha, China
- Wen Chen
- Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Changsha, China
- Wen Chen
- Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, China
- Wen Chen
- Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Changsha, China
- Wen Chen
- National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Changsha, China
- Wen Chen
- Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, China
- Fengna Li
- Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Changsha, China
- Fengna Li
- Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, China
- Fengna Li
- Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Changsha, China
- Fengna Li
- National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Changsha, China
- Fengna Li
- Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, China
- DOI
- https://doi.org/10.3389/fphys.2020.00449
- Journal volume & issue
-
Vol. 11
Abstract
Background: Intestinal barrier contributes as an important role in maintaining intestinal homeostasis. Oxidative stress can cause critical damages in intestinal integrity of animals.Objectives: This study was conducted to investigate the alleviated effect of taurine against small intestine (duodenum, jejunum, ileum) injury induced by oxidative stress.Methods: The piglet model of diquat-induced oxidative stress was employed. In addition, analysis of intestinal morphology, reverse transcription PCR (RT-PCR), and Western blot were used in this study.Results: Compared with the control group (CON), diquat-induced oxidative stress triggers immune response; the content of immunoglobulin M (IgM) and immunoglobulin G (IgG) was significantly changed, but 0.60% taurine supplementation could restore the level of serum immunoglobulin. Oxidative stress induces serious damage in intestinal morphology structure and tight junction barrier. Compared with the CON, the villus height of intestine was significantly decreased, the crypt depth and villus height/crypt depth (V/C) were also decreased, and 0.60% taurine supplementation could restore impaired morphology and even improve crypt depth and V/C of the jejunum and ileum. Compared with the CON, oxidative stress markedly increased the messenger RNA (mRNA) expression level of claudin-1 and occludin in the duodenum, and the value of occludin was significantly decreased in the jejunum of the diquat group (DIQ). Relative to the DIQ, 0.60% taurine supplementation increased the mRNA expression level of claudin-1, occludin, and ZO-1 in the ileum. Compared with the CON, the expression of claudin-1 protein was significantly upregulated, and occludin and ZO-1 protein were both downregulated in the small intestine of DIQ.Conclusion: Taurine exerts protective effects by regulating immune response and restores the intestinal tight junction barrier when piglets suffer from oxidative stress.
Keywords
