Nature Communications (Sep 2016)

Coordinated nuclease activities counteract Ku at single-ended DNA double-strand breaks

  • Pauline Chanut,
  • Sébastien Britton,
  • Julia Coates,
  • Stephen P. Jackson,
  • Patrick Calsou

DOI
https://doi.org/10.1038/ncomms12889
Journal volume & issue
Vol. 7, no. 1
pp. 1 – 12

Abstract

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Homologous recombination requires end resection of the DNA at the site of the break, however the Ku dimer can sequester single-ended double-strand breaks. Here the authors show that ATM-dependent phosphorylation of CtIP, along with the actions of Mre11, impair the stable loading of Ku onto DNA.