Generation, culture, and stimulation of small intestinal murine organoids in parasitology research

Abstract

Summary: Parasitic helminth worms frequently infect the gastrointestinal tract and interact with the intestinal epithelium and specialized cell types within it. Intestinal organoids derived from stem cells that line the intestine represent a transformational technology in the study of epithelial-parasite dialogue. Here, we present a protocol for establishing small intestine organoid cultures and administering parasite products of interest to these cultures. We then describe steps for evaluating their impact by microscopy, flow cytometry, immunohistology, and mRNA gene expression.For complete details on the use and execution of this protocol, please refer to Drurey et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• Flow Cytometry/Mass Cytometry
• Immunology
• Microscopy
• Gene Expression
• Organoids