3D-printed Recoverable Microdrive and Base Plate System for Rodent Electrophysiology
Mihaly Vöröslakos,
HIroyuki Miyawaki,
Sebastien Royer,
Kamran Diba,
Euisik Yoon,
Peter Petersen,
György Buzsáki
Affiliations
Mihaly Vöröslakos
Neuroscience Institute, Langone Medical Center, New York University, New York, NY 10016, United StatesDepartment of Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, MI, 48109, USA
HIroyuki Miyawaki
Department of Physiology, Osaka City University Graduate School of Medicine, Asahimachi 1-4-3, Abeno-ku, Osaka, 545-8585, Japan
Sebastien Royer
Center for Functional Connectomics, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea
Kamran Diba
Department of Anesthesiology, University of Michigan, Ann Arbor, MI 48109, USA
Euisik Yoon
Department of Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, MI, 48109, USADepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA, Center for Nanomedicine, Institute for Basic Science (IBS) and Graduate Program of Nano Biomedical Engineering (Nano BME), Yonsei University, Seoul, Korea
Peter Petersen
Neuroscience Institute, Langone Medical Center, New York University, New York, NY 10016, USA
György Buzsáki
Neuroscience Institute, Langone Medical Center, New York University, New York, NY 10016, USADepartment of Neurology, Langone Medical Center, New York University, New York, NY 10016, USA
Extracellular recordings in freely moving animals allow the monitoring of brain activity from populations of neurons at single-spike temporal resolution. While state-of-the-art electrophysiological recording devices have been developed in recent years (e.g., µLED and Neuropixels silicon probes), implantation methods for silicon probes in rats and mice have not advanced substantially for a decade. The surgery is complex, takes time to master, and involves handling expensive devices and valuable animal subjects. In addition, chronic silicon neural probes are practically single implant devices due to the current low success rate of probe recovery. To successfully recover silicon probes, improve upon the quality of electrophysiological recording, and make silicon probe recordings more accessible, we have designed a miniature, low cost, and recoverable microdrive system. The addition of a novel 3D-printed skull baseplate makes the surgery less invasive, faster, and simpler for both rats and mice. We provide detailed procedural instructions and print designs, allowing researchers to adapt and flexibly customize our designs to their experimental usage.
