Cancer Management and Research (Aug 2021)
Aberration of lncRNA LINC00460 is a Promising Prognosis Factor and Associated with Progression of Clear Cell Renal Cell Carcinoma
Abstract
Shijie Zhang,1 Fengyun Zhang,2 Yingdong Niu,3 Shenglong Yu4 1General Surgery, The Second Affiliated Hospital of Mudanjiang Medical University, Mudanjiang, Heilongjiang, 157011, People’s Republic of China; 2Hematology and Rheumatology, The Second Affiliated Hospital of Mudanjiang Medical University, Mudanjiang, Heilongjiang, 157011, People’s Republic of China; 3Supply Room, The Second Affiliated Hospital of Mudanjiang Medical University, Mudanjiang, Heilongjiang, 157011, People’s Republic of China; 4Urology Surgery, The Second Affiliated Hospital of Mudanjiang Medical University, Mudanjiang, Heilongjiang, 157011, People’s Republic of ChinaCorrespondence: Shenglong YuUrology Surgery, The Second Affiliated Hospital of Mudanjiang Medical University, No. 15 Dongxiaoyun Street, Aimin District, Mudanjiang, Heilongjiang, 157011, People’s Republic of ChinaTel +86-13945395825Email [email protected]: Long noncoding RNAs have been studied more and more as potential prognostic markers. However, the prognostic of LINC00460 in clear cell renal cell carcinoma (ccRCC) has not been explored. In this study, the potential role of LINC00460 was investigated in ccRCC.Patients and Methods: One hundred thirteen pairs of ccRCC tissues and para-normal tissues were collected. The expressions of LINC00460 in these tissues and ccRCC cells were evaluated via qRT-PCR. The prognostic value of LINC00460 was accessed with the use of Kaplan–Meier analysis and Cox proportional hazards model analysis. The influence of LINC00460 on ccRCC cell proliferation, migration, and invasion was determined via cell counting kit-8 (CCK-8) and Transwell assays.Results: The results revealed that LINC00460 was significantly enhanced in ccRCC tissues, as well as in ccRCC cell lines. The overexpression of LINC00460 was significantly associated with lymph node metastasis and TNM stage, and lead to poor overall survival. Knockdown of LINC00460 reduces the cell ability of proliferation, migration, and invasion. LINC00460 could sponge to miR-149-5p.Conclusion: LINC00460 may be developed as a prognostic biomarker and molecular therapy target for ccRCC.Keywords: LINC00460, clear cell renal cell carcinoma, miR-149-5p, prognosis
