Lactylome analysis reveals potential target modified proteins in the retina of form-deprivation myopia
Jiaojiao Feng,
Xiaoniao Chen,
Runkuan Li,
Yunxiao Xie,
Xiuyan Zhang,
Xiaoxiao Guo,
Lianghui Zhao,
Zhe Xu,
Yifan Song,
Jike Song,
Hongsheng Bi
Affiliations
Jiaojiao Feng
Shandong University of Traditional Chinese Medicine, Jinan 250014, China
Xiaoniao Chen
Department of Ophthalmology, Chinese PLA General Hospital, Beijing 100853, China
Runkuan Li
Shandong University of Traditional Chinese Medicine, Jinan 250014, China
Yunxiao Xie
Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250002, China; Shandong Academy of Eye Disease Prevention and Therapy, Shandong Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases, Jinan 250002, China
Xiuyan Zhang
Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250002, China; Shandong Academy of Eye Disease Prevention and Therapy, Shandong Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases, Jinan 250002, China
Xiaoxiao Guo
Shandong University of Traditional Chinese Medicine, Jinan 250014, China
Lianghui Zhao
Shandong University of Traditional Chinese Medicine, Jinan 250014, China
Zhe Xu
Shandong University of Traditional Chinese Medicine, Jinan 250014, China
Yifan Song
Shandong University of Traditional Chinese Medicine, Jinan 250014, China
Jike Song
Shandong University of Traditional Chinese Medicine, Jinan 250014, China; Shandong Academy of Eye Disease Prevention and Therapy, Shandong Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases, Jinan 250002, China; Corresponding author
Hongsheng Bi
Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250002, China; Shandong Academy of Eye Disease Prevention and Therapy, Shandong Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases, Jinan 250002, China; Corresponding author
Summary: The biological mechanisms underlying the development of myopia have not yet been completely elucidated. The retina is critical for visual signal processing, which primarily utilizes aerobic glycolysis to produce lactate as a metabolic end product. Lactate facilitates lysine lactylation (Kla), a posttranslational modification essential for transcriptional regulation. This study found increased glycolytic flux and lactate accumulation in the retinas of form-deprived myopic guinea pigs. Subsequently, a comprehensive analysis of Kla levels in retinal proteins revealed that Kla was upregulated at 124 sites in 92 proteins and downregulated at three sites in three proteins. Functional enrichment and protein interaction analyses showed significant enrichment in pathways related to energy metabolism, including glutathione metabolism, glycolysis, and the hypoxia-inducible factor-1 signaling pathway. Parallel-reaction monitoring confirmed data reliability. These findings suggest a connection between myopia and retinal energy metabolism imbalance, providing new insights into the pathogenesis of myopia.
