Summary: Parvalbumin (PV)-expressing GABAergic neurons are the largest class of inhibitory neocortical cells. We visualize brain-wide, monosynaptic inputs to PV neurons in mouse barrel cortex. We develop intersectional rabies virus tracing to specifically target GABAergic PV cells and exclude a small fraction of excitatory PV cells from our starter population. Local inputs are mainly from layer (L) IV and excitatory cells. A small number of inhibitory inputs originate from LI neurons, which connect to LII/III PV neurons. Long-range inputs originate mainly from other sensory cortices and the thalamus. In visual cortex, most transsynaptically labeled neurons are located in LIV, which contains a molecularly mixed population of projection neurons with putative functional similarity to LIII neurons. This study expands our knowledge of the brain-wide circuits in which PV neurons are embedded and introduces intersectional rabies virus tracing as an applicable tool to dissect the circuitry of more clearly defined cell types. : Hafner et al. develop constructs for intersectional rabies virus tracing to map brain-wide, monosynaptic inputs to cortical inhibitory parvalbumin cells. They find extensive local innervation by excitatory cells, inputs from inhibitory cells in layer I, and a strong contribution of layer IV projection neurons to cortical long-range inputs. Keywords: parvalbumin, GABAergic neuron, barrel cortex, rabies tracing, intersectional, Cre, Flp
