PLoS ONE (Jan 2015)

Islet Oxygen Consumption Rate (OCR) Dose Predicts Insulin Independence in Clinical Islet Autotransplantation.

  • Klearchos K Papas,
  • Melena D Bellin,
  • David E R Sutherland,
  • Thomas M Suszynski,
  • Jennifer P Kitzmann,
  • Efstathios S Avgoustiniatos,
  • Angelika C Gruessner,
  • Kathryn R Mueller,
  • Gregory J Beilman,
  • Appakalai N Balamurugan,
  • Gopalakrishnan Loganathan,
  • Clark K Colton,
  • Maria Koulmanda,
  • Gordon C Weir,
  • Josh J Wilhelm,
  • Dajun Qian,
  • Joyce C Niland,
  • Bernhard J Hering

DOI
https://doi.org/10.1371/journal.pone.0134428
Journal volume & issue
Vol. 10, no. 8
p. e0134428

Abstract

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BackgroundReliable in vitro islet quality assessment assays that can be performed routinely, prospectively, and are able to predict clinical transplant outcomes are needed. In this paper we present data on the utility of an assay based on cellular oxygen consumption rate (OCR) in predicting clinical islet autotransplant (IAT) insulin independence (II). IAT is an attractive model for evaluating characterization assays regarding their utility in predicting II due to an absence of confounding factors such as immune rejection and immunosuppressant toxicity.MethodsMembrane integrity staining (FDA/PI), OCR normalized to DNA (OCR/DNA), islet equivalent (IE) and OCR (viable IE) normalized to recipient body weight (IE dose and OCR dose), and OCR/DNA normalized to islet size index (ISI) were used to characterize autoislet preparations (n = 35). Correlation between pre-IAT islet product characteristics and II was determined using receiver operating characteristic analysis.ResultsPreparations that resulted in II had significantly higher OCR dose and IE dose (pConclusionsCommonly used assays to determine whether a clinical islet preparation is of high quality prior to transplantation are greatly lacking in sensitivity and specificity. While IE dose is highly predictive, it does not take into account islet cell quality. OCR dose, which takes into consideration both islet cell quality and quantity, may enable a more accurate and prospective evaluation of clinical islet preparations.