Construction of a Bacillus subtilis and Escherichia coli shuttle vector harboring the fabL gene as a triclosan selection marker
Elena A. Mordukhova,
Jae-Gu Pan
Affiliations
Elena A. Mordukhova
GenoFocus Ltd., 65 Techno 1-ro, Gwanpyeong-dong, Yuseong-gu, Daejeon, 34014, South Korea
Jae-Gu Pan
GenoFocus Ltd., 65 Techno 1-ro, Gwanpyeong-dong, Yuseong-gu, Daejeon, 34014, South Korea; Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 111 Gwahangno, Yuseong, Daejeon, 34141, South Korea; Corresponding author.
A new plasmid containing a mutated fabL gene from Bacillus subtilis as a triclosan selection marker was developed as a useful B. subtilis/E. coli shuttle vector. The pHT-FabL40 plasmid is stable in both gram-positive and gram-negative hosts with increased plasmid DNA yield in E. coli.
