Comparison of three methods in the cultivation of cellular pendants derived from the Kalas, which is the technology of landfill in cattle

Abstract

The present study established cell suspensions from the friable callus derived from the stem explant of broad bean (Vi cia faba) axenic seedlings. These suspensions of certain densities were cultured by embedding in agar, using three methods of culture including, multiple drop arrays (MDA), thin layers and sectors. In the presence of MS andSH liquid medium forming solid - liquid medium. The results indicated that all methods succeeded at culturing cellsuspension using MS medium. Moreover, these methods sustained callus f01mation. Multiple Drop Array (MDA) induced callus at ratio of 68% in MS medium, and 48% in SH medium. All pieces of callus primordiacontinued their growth and increased in size.

Keywords

• cultivation
• landfill in cattle
• solid - liquid medium