The Plant Pathology Journal (Jun 2019)

Characterization of Burkholderia glumae Putative Virulence Factor 11 (PVF11) via Yeast Two-Hybrid Interaction and Phenotypic Analysis

  • Juyun Kim,
  • Namgyu Kim,
  • Mohamed Mannaa,
  • Hyun-Hee Lee,
  • Jong-Seong Jeon,
  • Young-Su Seo

DOI
https://doi.org/10.5423/PPJ.NT.02.2019.0026
Journal volume & issue
Vol. 35, no. 3
pp. 280 – 286

Abstract

Read online

In this study, PVF11 was selected among 20 candidate pathogenesis-related genes in Burkholderia glumae based on its effect on virulence to rice. PVF11 was found to interact with several plant defense-related WRKY proteins as evidenced through yeast-two hybrid analysis (Y2H). Moreover, PVF11 showed interactions with abiotic and biotic stress response-related rice proteins, as shown by genome-wide Y2H screening employing PVF11 and a cDNA library from B. glumae-infected rice. To confirm the effect of PVF11 on B. glumae virulence, in planta assays were conducted at different stages of rice growth. As a result, a PVF11-defective mutant showed reduced virulence in rice seedlings and stems but not in rice panicles, indicating that PVF11 involvement in B. glumae virulence in rice is stage-dependent.

Keywords