Generation of inflammation-responsive astrocytes from glial progenitors derived from human pluripotent stem cells

Renata Santos; Arianna Mei; Maria C. Marchetto

STAR Protocols (Jun 2022)

Generation of inflammation-responsive astrocytes from glial progenitors derived from human pluripotent stem cells

Renata Santos,
Arianna Mei,
Maria C. Marchetto

Affiliations

Renata Santos: Laboratory of Genetics, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA; Université Paris Cité, INSERM U1266, Institute of Psychiatry and Neuroscience of Paris, Laboratory of Dynamics of Neuronal Structure in Health and Disease, 102 rue de la Santé, 75014 Paris, France; Corresponding author
Arianna Mei: Laboratory of Genetics, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
Maria C. Marchetto: Laboratory of Genetics, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA; Department of Anthropology, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA; Corresponding author

Journal volume & issue: Vol. 3, no. 2
p. 101261

Abstract

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Summary: Astrocytes are an essential component of the central nervous system for neuronal support and response to injury and disease. Here, we present a protocol to generate glial progenitor cells (GPCs) from human-induced pluripotent stem cells (iPSCs) that can be further differentiated into inflammation-responsive astrocytes. This two-step protocol has the advantage of reducing the time of astrocyte differentiation since GPCs can be frozen and stored.For complete details on the use and execution of this protocol, please refer to Santos et al. (2017) and Vadodaria et al. (2021).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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