Communications Biology (Dec 2021)

In vitro functional analysis of gRNA sites regulating assembly of hepatitis B virus

  • Nikesh Patel,
  • Sam Clark,
  • Eva U. Weiß,
  • Carlos P. Mata,
  • Jen Bohon,
  • Erik R. Farquhar,
  • Daniel P. Maskell,
  • Neil A. Ranson,
  • Reidun Twarock,
  • Peter G. Stockley

DOI
https://doi.org/10.1038/s42003-021-02897-2
Journal volume & issue
Vol. 4, no. 1
pp. 1 – 12

Abstract

Read online

Patel et al. investigate the role of RNA sequence/structure motif ‘Packaging Signals’ (PSs) in regulating the assembly of an HBV genome transcript using an in vitro assay containing only core protein (Cp) and RNA. They show that RNA PSs regulate assembly of HBV genomic transcripts in vitro, and therefore may play similar roles in vivo, in concert with other molecular factors.