Nature Communications (Jan 2022)

Structural mechanism for tyrosine hydroxylase inhibition by dopamine and reactivation by Ser40 phosphorylation

  • María Teresa Bueno-Carrasco,
  • Jorge Cuéllar,
  • Marte I. Flydal,
  • César Santiago,
  • Trond-André Kråkenes,
  • Rune Kleppe,
  • José R. López-Blanco,
  • Miguel Marcilla,
  • Knut Teigen,
  • Sara Alvira,
  • Pablo Chacón,
  • Aurora Martinez,
  • José M. Valpuesta

DOI
https://doi.org/10.1038/s41467-021-27657-y
Journal volume & issue
Vol. 13, no. 1
pp. 1 – 16

Abstract

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Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the synthesis of the catecholamine neurotransmitters and hormones dopamine (DA), adrenaline and noradrenaline. Here, the authors present the cryo-EM structures of full-length human TH in the apo form and bound with DA, as well as the structure of Ser40 phosphorylated TH, and discuss the inhibitory and stabilizing effects of DA on TH and its counteraction by Ser40-phosphorylation.