Nature Communications (Mar 2016)
Cyclophilin A stabilizes the HIV-1 capsid through a novel non-canonical binding site
- Chuang Liu,
- Juan R. Perilla,
- Jiying Ning,
- Manman Lu,
- Guangjin Hou,
- Ruben Ramalho,
- Benjamin A. Himes,
- Gongpu Zhao,
- Gregory J. Bedwell,
- In-Ja Byeon,
- Jinwoo Ahn,
- Angela M. Gronenborn,
- Peter E. Prevelige,
- Itay Rousso,
- Christopher Aiken,
- Tatyana Polenova,
- Klaus Schulten,
- Peijun Zhang
Affiliations
- Chuang Liu
- Department of Structural Biology, University of Pittsburgh School of Medicine
- Juan R. Perilla
- Department of Physics and Beckman Institute, University of Illinois at Urbana-Champaign
- Jiying Ning
- Department of Structural Biology, University of Pittsburgh School of Medicine
- Manman Lu
- Pittsburgh Center for HIV Protein Interactions, University of Pittsburgh School of Medicine
- Guangjin Hou
- Pittsburgh Center for HIV Protein Interactions, University of Pittsburgh School of Medicine
- Ruben Ramalho
- Department of Physiology and Cell Biology, Ben-Gurion University of the Negev
- Benjamin A. Himes
- Department of Structural Biology, University of Pittsburgh School of Medicine
- Gongpu Zhao
- Department of Structural Biology, University of Pittsburgh School of Medicine
- Gregory J. Bedwell
- Department of Microbiology, University of Alabama at Birmingham
- In-Ja Byeon
- Department of Structural Biology, University of Pittsburgh School of Medicine
- Jinwoo Ahn
- Department of Structural Biology, University of Pittsburgh School of Medicine
- Angela M. Gronenborn
- Department of Structural Biology, University of Pittsburgh School of Medicine
- Peter E. Prevelige
- Pittsburgh Center for HIV Protein Interactions, University of Pittsburgh School of Medicine
- Itay Rousso
- Department of Physiology and Cell Biology, Ben-Gurion University of the Negev
- Christopher Aiken
- Pittsburgh Center for HIV Protein Interactions, University of Pittsburgh School of Medicine
- Tatyana Polenova
- Pittsburgh Center for HIV Protein Interactions, University of Pittsburgh School of Medicine
- Klaus Schulten
- Department of Physics and Beckman Institute, University of Illinois at Urbana-Champaign
- Peijun Zhang
- Department of Structural Biology, University of Pittsburgh School of Medicine
- DOI
- https://doi.org/10.1038/ncomms10714
- Journal volume & issue
-
Vol. 7,
no. 1
pp. 1 – 10
Abstract
The host cell factor cyclophilin A (CypA) interacts with the HIV-1 capsid and regulates infectivity. Here the authors combine cryo-EM, solid-state NMR and all-atom MD simulations, identifying an interaction interface between CypA and the HIV capsid that stabilizes the viral capsid and regulates infectivity.
