Magnetic Mixed Micelles Composed of a Non-Ionic Surfactant and Nitroxide Radicals Containing a D-Glucosamine Unit: Preparation, Stability, and Biomedical Application
Kota Nagura,
Yusa Takemoto,
Fumi Yoshino,
Alexey Bogdanov,
Natalia Chumakova,
Andrey Kh. Vorobiev,
Hirohiko Imai,
Tetsuya Matsuda,
Satoshi Shimono,
Tatsuhisa Kato,
Naoki Komatsu,
Rui Tamura
Affiliations
Kota Nagura
Graduate School of Human and Environmental Studies, Kyoto University, Kyoto 606-8501, Japan
Yusa Takemoto
Graduate School of Human and Environmental Studies, Kyoto University, Kyoto 606-8501, Japan
Fumi Yoshino
Department of Obstetrics and Gynecology, Shiga University of Medical Science, Shiga 520-2192, Japan
Alexey Bogdanov
Department of Chemistry, M.V. Lomonosov Moscow State University, Moscow 119991, Russian Federation
Natalia Chumakova
Department of Chemistry, M.V. Lomonosov Moscow State University, Moscow 119991, Russian Federation
Andrey Kh. Vorobiev
Department of Chemistry, M.V. Lomonosov Moscow State University, Moscow 119991, Russian Federation
Hirohiko Imai
Graduate School of Informatics, Kyoto University, Kyoto 606-8501, Japan
Tetsuya Matsuda
Graduate School of Informatics, Kyoto University, Kyoto 606-8501, Japan
Satoshi Shimono
Graduate School of Human and Environmental Studies, Kyoto University, Kyoto 606-8501, Japan
Tatsuhisa Kato
Graduate School of Human and Environmental Studies, Kyoto University, Kyoto 606-8501, Japan
Naoki Komatsu
Graduate School of Human and Environmental Studies, Kyoto University, Kyoto 606-8501, Japan
Rui Tamura
Graduate School of Human and Environmental Studies, Kyoto University, Kyoto 606-8501, Japan
Metal-free magnetic mixed micelles (mean diameter: < 20 nm) were prepared by mixing the biocompatible non-ionic surfactant Tween 80 and the non-toxic, hydrophobic pyrrolidine-N-oxyl radicals bearing a D-glucosamine unit in pH 7.4 phosphate-buffered saline (PBS). The time-course stability and in vitro magnetic resonance imaging (MRI) contrast ability of the mixed micelles was found to depend on the length of the alkyl chain in the nitroxide radicals. It was also confirmed that the mixed micelles exhibited no toxicity in vivo and in vitro and high stability in the presence of a large excess of ascorbic acid. The in vivo MRI experiment revealed that one of these mixed micelles showed much higher contrast enhancement in the proton longitudinal relaxation time (T1) weighted images than other magnetic mixed micelles that we have reported previously. Thus, the magnetic mixed micelles presented here are expected to serve as a promising contrast agent for theranostic nanomedicines, such as MRI-visible targeted drug delivery carriers.
