Nature Communications (Feb 2016)
Mechanisms of amphetamine action illuminated through optical monitoring of dopamine synaptic vesicles in Drosophila brain
- Zachary Freyberg,
- Mark S. Sonders,
- Jenny I. Aguilar,
- Takato Hiranita,
- Caline S. Karam,
- Jorge Flores,
- Andrea B. Pizzo,
- Yuchao Zhang,
- Zachary J. Farino,
- Audrey Chen,
- Ciara A. Martin,
- Theresa A. Kopajtic,
- Hao Fei,
- Gang Hu,
- Yi-Ying Lin,
- Eugene V. Mosharov,
- Brian D. McCabe,
- Robin Freyberg,
- Kandatege Wimalasena,
- Ling-Wei Hsin,
- Dalibor Sames,
- David E. Krantz,
- Jonathan L. Katz,
- David Sulzer,
- Jonathan A. Javitch
Affiliations
- Zachary Freyberg
- Department of Psychiatry, College of Physicians & Surgeons, Columbia University
- Mark S. Sonders
- Department of Psychiatry, College of Physicians & Surgeons, Columbia University
- Jenny I. Aguilar
- Department of Psychiatry, College of Physicians & Surgeons, Columbia University
- Takato Hiranita
- Department of Health and Human Services, Psychobiology Section, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health
- Caline S. Karam
- Department of Psychiatry, College of Physicians & Surgeons, Columbia University
- Jorge Flores
- Department of Biochemistry and Molecular Biophysics, Howard Hughes Medical Institute, College of Physicians & Surgeons, Columbia University
- Andrea B. Pizzo
- Department of Psychiatry, College of Physicians & Surgeons, Columbia University
- Yuchao Zhang
- Department of Psychiatry, College of Physicians & Surgeons, Columbia University
- Zachary J. Farino
- Department of Psychiatry, College of Physicians & Surgeons, Columbia University
- Audrey Chen
- Department of Psychiatry and Biobehavioral Sciences and Semel Institute for Neuroscience and Human Behavior, Hatos Center for Neuropharmacology, David Geffen School of Medicine University of California
- Ciara A. Martin
- UCLA Interdepartmental Program in Molecular Toxicology, University of California
- Theresa A. Kopajtic
- Department of Health and Human Services, Psychobiology Section, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health
- Hao Fei
- Department of Psychiatry and Biobehavioral Sciences and Semel Institute for Neuroscience and Human Behavior, Hatos Center for Neuropharmacology, David Geffen School of Medicine University of California
- Gang Hu
- Department of Chemistry, Columbia University
- Yi-Ying Lin
- School of Pharmacy, College of Medicine, National Taiwan University
- Eugene V. Mosharov
- Department of Neurology, College of Physicians & Surgeons, Columbia University
- Brian D. McCabe
- Center for Motor Neuron Biology and Disease, College of Physicians & Surgeons, Columbia University
- Robin Freyberg
- Department of Psychology, Stern College for Women, Yeshiva University
- Kandatege Wimalasena
- Department of Chemistry, Wichita State University
- Ling-Wei Hsin
- School of Pharmacy, College of Medicine, National Taiwan University
- Dalibor Sames
- Department of Chemistry, Columbia University
- David E. Krantz
- Department of Psychiatry and Biobehavioral Sciences and Semel Institute for Neuroscience and Human Behavior, Hatos Center for Neuropharmacology, David Geffen School of Medicine University of California
- Jonathan L. Katz
- Department of Health and Human Services, Psychobiology Section, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health
- David Sulzer
- Department of Psychiatry, College of Physicians & Surgeons, Columbia University
- Jonathan A. Javitch
- Department of Psychiatry, College of Physicians & Surgeons, Columbia University
- DOI
- https://doi.org/10.1038/ncomms10652
- Journal volume & issue
-
Vol. 7,
no. 1
pp. 1 – 15
Abstract
Amphetamines are known to enhance extracellular dopamine levels, but the underlying mechanisms are unclear. Utilising a new pH biosensor for synaptic vesicles, the authors show that amphetamines diminish vesicle pH gradients, disrupting dopamine packaging and leading to increased neurotransmitter release.
