Detection and Discrimination of Non-Melanoma Skin Cancer by Multimodal Imaging

Healthcare. 2013;1(1):64-83 DOI 10.3390/healthcare1010064


Journal Homepage

Journal Title: Healthcare

ISSN: 2227-9032 (Online)

Publisher: MDPI AG

LCC Subject Category: Medicine

Country of publisher: Switzerland

Language of fulltext: English

Full-text formats available: PDF, HTML, XML



Jürgen Popp
Benjamin Dietzek
Jürgen Lademann
Hans-Joachim Röwert-Huber
Franziska Kluschke
Tobias Meyer
Denis Akimov
Sandro Heuke
Nadine Vogler


Blind peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 11 weeks


Abstract | Full Text

Non-melanoma skin cancer (NMSC) belongs to the most frequent human neoplasms. Its exposed location facilitates a fast ambulant treatment. However, in the clinical practice far more lesions are removed than necessary, due to the lack of an efficient pre-operational examination procedure: Standard imaging methods often do not provide a sufficient spatial resolution. The demand for an efficient in vivo imaging technique might be met in the near future by non-linear microscopy. As a first step towards this goal, the appearance of NMSC in various microspectroscopic modalities has to be defined and approaches have to be derived to distinguish healthy skin from NMSC using non-linear optical microscopy. Therefore, in this contribution the appearance of ex vivo NMSC in a combination of coherent anti-Stokes Raman scattering (CARS), second harmonic generation (SHG) and two photon excited fluorescence (TPEF) imaging—referred as multimodal imaging—is described. Analogous to H&E staining, an overview of the distinct appearances and features of basal cell and squamous cell carcinoma in the complementary modalities is derived, and is expected to boost in vivo studies of this promising technological approach.