Single-molecule trapping and spectroscopy reveals photophysical heterogeneity of phycobilisomes quenched by Orange Carotenoid Protein

Allison H. Squires; Peter D. Dahlberg; Haijun Liu; Nikki Cecil M. Magdaong; Robert E. Blankenship; W. E. Moerner

doi:10.1038/s41467-019-09084-2

Nature Communications (Mar 2019)

Single-molecule trapping and spectroscopy reveals photophysical heterogeneity of phycobilisomes quenched by Orange Carotenoid Protein

Allison H. Squires,
Peter D. Dahlberg,
Haijun Liu,
Nikki Cecil M. Magdaong,
Robert E. Blankenship,
W. E. Moerner

Affiliations

Allison H. Squires: Department of Chemistry, Stanford University
Peter D. Dahlberg: Department of Chemistry, Stanford University
Haijun Liu: Departments of Biology and Chemistry, Washington University in St. Louis
Nikki Cecil M. Magdaong: Departments of Biology and Chemistry, Washington University in St. Louis
Robert E. Blankenship: Departments of Biology and Chemistry, Washington University in St. Louis
W. E. Moerner: Department of Chemistry, Stanford University

DOI: https://doi.org/10.1038/s41467-019-09084-2
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 12

Abstract

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Upon photoactivation the Orange Carotenoid Protein (OCP) binds to the phycobilisome and prevents damage by thermally dissipating excess energy. Here authors use an Anti-Brownian ELectrokinetic trap to determine the photophysics of single OCP-quenched phycobilisomes and observe two distinct OCP-quenched states with either one or two OCPs bound.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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