Stem Cell Research (Nov 2015)

Generation of integration-free induced pluripotent stem cells from a patient with Familial Mediterranean Fever (FMF)

  • Kerem Fidan,
  • Gülnihal Kavaklıoğlu,
  • Ayyub Ebrahimi,
  • Can Özlü,
  • Nur Zeynep Ay,
  • Arzu Ruacan,
  • Ahmet Gül,
  • Tamer T. Önder

DOI
https://doi.org/10.1016/j.scr.2015.10.018
Journal volume & issue
Vol. 15, no. 3
pp. 694 – 696

Abstract

Read online

Fibroblasts from a Familial Mediterranean Fever (FMF) patient were reprogrammed with episomal vectors by using the Neon Transfection System for the generation of integration-free induced pluripotent stem cells (iPSCs). The resulting iPSC line was characterized to determine the expression of pluripotency markers, proper differentiation into three germ layers, the presence of normal chromosomal structures as well as the lack of genomic integration. A homozygous missense mutation in the MEFV gene (p.Met694Val), which lead to typical FMF phenotype, was shown to be present in the generated iPSC line.