BMC Research Notes (Nov 2019)

Proteomic analysis of Nrk gene-disrupted placental tissue cells explains physiological significance of NRK

  • Kimitoshi Denda,
  • Kanako Ida,
  • Masataka Tanno,
  • Kanako Nakao-Wakabayashi,
  • Masayuki Komada,
  • Nobuhiro Hayashi

DOI
https://doi.org/10.1186/s13104-019-4818-7
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 5

Abstract

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Abstract Objective NRK is a unique X chromosome-linked protein kinase expressed predominantly in placenta. The gene knockout causes placental overgrowth and delayed labor of Nrk-null fetuses from dams in mouse. To clarify unknown mechanisms behind the Nrk-null phenotypes, protein expression profiles were analyzed in the Nrk-null placenta using a high-performance two-dimensional electrophoresis methodology. Results Among around 1800 spots detected, we characterized a dozen protein spots whose expression levels were significantly altered in the Nrk-null placenta compared to wild-type. Analyzing these data sets is expected to reflect the difference physiologically in the presence or absence of NRK, facilitating the development of therapeutic strategies.

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