Development and Mapping of Microsatellite Markers Derived from cDNA in Japanese Quail (Coturnix japonica)

Abstract

The objective of this work was to develop polymorphic microsatellite markers derived from Japanese quail embryonic and cardiac cDNA libraries and to locate these markers on Kobe-NIBS Japanese quail (KNQ) linkage map constructed mainly by AFLP markers in previous study. Of the 86 positive clones, 51 clones were unique and identified as genes or ESTs by BLAST searches, and 25 from 51 clones were possible to design the PCR primers after partial sequencing. Only six markers were polymorphic and used by linkage analysis in KNQ resource family. These markers were mapped on four linkage groups, JQG 1, JQG 9, JQG 19 and JQG 66, and then these linkage groups were assigned to chromosome of Japanese quail, CJA 2, CJA 14, CJA 17 and CJA 23, respectively. These markers derived from cDNA are useful and powerful tool, and contribute to the comparative map between Japanese quail and the other species.

Keywords

• comparative mapping
• functional gene
• japanese quail
• linkage map
• microsatellite