Nature Communications (Mar 2020)

Structure of E3 ligase E6AP with a proteasome-binding site provided by substrate receptor hRpn10

  • Gwen R. Buel,
  • Xiang Chen,
  • Raj Chari,
  • Maura J. O’Neill,
  • Danielle L. Ebelle,
  • Conor Jenkins,
  • Vinidhra Sridharan,
  • Sergey G. Tarasov,
  • Nadya I. Tarasova,
  • Thorkell Andresson,
  • Kylie J. Walters

DOI
https://doi.org/10.1038/s41467-020-15073-7
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 15

Abstract

Read online

Human ubiquitin E3 ligase E6AP contains a Zn-binding AZUL domain. Here the authors identify and name RAZUL, a domain in proteasome substrate receptor hRpn10 that binds AZUL, recruiting E6AP to proteasomes, and they present the NMR structure of the RAZUL:AZUL complex, which forms an intermolecular 4-helix bundle.