Effects of FeSO₄ and Organic Additives on Soil Properties and Microbiota during Model Soybean Planting in Saline-Alkali Soil

Abstract

Saline soils are characterized by organic matter and nutrient deficiencies, and their mineral fraction consists almost exclusively of fine sand particles, resulting in an unstable soil formation process. Due to the high amount of soluble salts in the soil, the osmotic pressure of the soil is elevated, restricting water absorption. This ultimately leads to the death of the plant and adversely impacts crop growth and yield. Incorporating Fe2+ can improve fertilizer utilization efficiency by reducing the oxidation of NH4+ to nitrogen (N2). However, reports on the usage of iron addition for the improvement of saline-alkali soils are scanty. This study conducted an outdoor simulation in pots to assess the soils of soybean crops during the podding stage. The effects of Fe2+ along with organic fertilizer or bio-C addition were elucidated on the composition and function of saline and alkaline microbial communities. The findings were correlated with soil environmental factors to analyze the dynamic changes in soil microbial communities. The soil pH decreased by 1.22–2.18% and SOM increased by 2.87–11.77% with organic fertilizer (OF) treatment. Compared to the ck treatment (control without iron supplementation), other treatments showed an average increase in abundance of dominant phylum by 8.25–11.23%, and an increase in the diversity and richness of the microbial community by 1.73–10.87%. The harmful bacteria in the Actinobacteriota, Chloroflexi, and Basidiomycota groups reduced by 57.83%, 74.29%, and 67.29%, and the beneficial bacteria in Ascomycota increased by 18.23–20.39%. Fe2+ combined with organic fertilizer or bio-C treatment could weaken the competitive relationship between the various bacterial lineages, enhance synergistic ability, favor the function and structure of the microbial community, and thus, improve the soil environment. Overall, the application of Fe2+ combined with organic fertilizers improved the saline-alkali soil, while the biochar (C) treatment mainly affected the soil nutrients. Through its detailed analysis, the study provides actionable insights for farmers to manage soil fertility in saline-alkaline soils, thereby overcoming the challenges of poor yields due to salinity stress. This will lead to resilient and sustainable farming systems, contributing to global food security.

Keywords

• organic matter
• soil physiochemical properties
• bacterial and fungal diversity
• network analysis