Cancer Management and Research (Oct 2020)

Oncolytic Vaccinia Virus-Mediated Antitumor Effect and Cell Proliferation Were Promoted in PTC by Regulating circRNA_103598/miR-23a-3p/IL-6 Axis

  • Zhang S,
  • Wang Q,
  • Li D,
  • Huang B,
  • Hou X,
  • Wang D,
  • Xiao Z,
  • Meng H,
  • Zhang Y,
  • Dong L

Journal volume & issue
Vol. Volume 12
pp. 10389 – 10396

Abstract

Read online

Shuilong Zhang,1 Qiang Wang,1 Dewei Li,1 Bo Huang,2 Xia Hou,3 Dongliang Wang,1 Zhifu Xiao,1 Hongjing Meng,1 Yuhang Zhang,1 Lingxiang Dong1 1Department of Thyroid Surgery, Shanxi Provincial People’s Hospital, Taiyuan, People’s Republic of China; 2Department of Gastrointestinal Pancreas, Hernia and Abdominal Wall Surgery, Shanxi Provincial People’s Hospital, Taiyuan, People’s Republic of China; 3Department of Chest Radiotherapy, Tumor Hospital of Shanxi Province, Taiyuan, People’s Republic of ChinaCorrespondence: Shuilong ZhangDepartment of Thyroid Surgery, Shanxi Provincial People’s Hospital, No. 29 Shuangtasi Street, Taiyuan, Shanxi 030012, People’s Republic of ChinaTel +86-0351-4960327Email [email protected]: Papillary thyroid carcinoma (PTC) is the most common type of thyroid malignancy, and cases have been rising steadily worldwide in the past few decades. Despite great progress having been made in surgery and chemotherapy for PTC, the survival rate of PTC patients has not increased significantly. Therefore, there is an urgent need to explore novel treatment strategies.Materials and Methods: The levels of circRNA_103598, miR-23a-3p and IL-6 mRNA in PTC tissues and cells were examined by qRT-PCR assay. Cell proliferation and IC50 values of oncolytic vaccinia virus (OVV) were detected by CCK-8 assay. A dual-luciferase reporter assay was performed to detect the relationships among circRNA_103598, miR-23a-3p and IL-6. ELISA was carried out to detect the expression of IL-6.Results: We found that circRNA_103598 was increased in PTC tissues and cell lines and acted as a sponge for miR-23a-3p. Moreover, knockdown of miR-23a-3p suppressed the OVV-mediated antitumor effect and cell proliferation in PTC. In addition, we revealed that circRNA_103598 bound to miR-23a-3p as a sponge to promote IL-6 expression.Conclusion: Our study first revealed the high expression and oncogenic function of circRNA_103598 in PTC cells. Then, circRNA_103598 sponged miR-23a-3p to upregulate IL-6 expression, with the resulted that cell proliferation was promoted and the OVV-mediated antitumor effect was enhanced by strengthening the viral replication, providing new insights into future therapy for PTC.Keywords: OVV, cell proliferation, circRNA_103598, miR-23a-3p, IL-6, PTC

Keywords