Journal of Kerman University of Medical Sciences (Mar 1997)
Induction of experimental allergic asthma in cat
Abstract
19 cats were nper-sensitized to allergic asthma by receiving a concomitant spray ang injection of ovalbumin (10% fresh egg-white in normal saline) every other days for four weeks.then animals were anesthetized and left femoral artery cannulation performed to record atrerial pressure and take blood samples.left femoral vein was cannulated to infuse nutritional fluids and grugs as needed.trachea was intubated for artificial ventilation and induction of experimental asthma by exposing pulmonary airways to allergen.a small perforation was performed in the chest wall between the 4-5 ribs in the right side and a special cannula placed into the pleural space to make a ercird of respiratory rate and plural pressure.the chest then was closed airtight and the animal was left at rest for three hours.experiment then started by spraying 1 ml/kg of allergen into the tracheal tube.at the end of the study the animal was killed by iv injection of saturated KCI,THE LUNG EXTRACTED AND SAVED IN 10% formaldehyde solution for histopathological studie.results of this study indicated that we could induce severe experimental asthma in the cat with clinical symptoms of severe hypoxemia,increased respiratory rate and sallow breathing (250% increase in rate and 64% decrease in pleural pressure as compared to control groyp:n=9, saline induced instead of allergen).pathologic signs of asthma was also observed by seeing air way constriction,distorted shape and presence of eosinophil and lymphocytes in air ways and in pulmonary parencchyma.therefero,it is concluded that with the above technique we could have induce allergic asthma in the cat.
