Possibility of In-House Preparation of Liver Cancer Diagnostic Kits Based on AFP ELISA Test

Abstract

Our research was focused on the study of possibility of preparation the AFP Enzyme-Linked Immunosorbent Assay (ELISA) diagnostic kits using tumor marker protein named alpha-fetoprotein (AFP) for hepatocellular carcinoma (HCC), the most frequent cancer in Mongolia. It is important to prepare the diagnostic kits for detection of liver cancer early, simply and inexpensively in this country. Detection of the marker in human sera would signifi cantly help successful therapy, since the tumor could be tackled at an early stage in its development, often before metastases and other consequences. Therefore, level of AFP in sera would give possibility to control the progression of liver cancer. The micro titer plates were prepared for AFP ELISA diagnostic kits, and their sensitivity and specifi city were 94.4% and 0.4, respectively. Statistically, there were no differences between our prepared micro titer plates for AFP ELISA diagnostic kits and commercially available ones ( t = 0.071, P = 0.94, df = 24). It was concluded that there is a possibility to prepare AFP ELISA diagnostic kits using our prepared micro titer plates in house.

Keywords

• AFP
• hepatocellular carcinoma
• ELISA