DNA origami scaffold for studying intrinsically disordered proteins of the nuclear pore complex

Philip Ketterer; Adithya N. Ananth; Diederik S. Laman Trip; Ankur Mishra; Eva Bertosin; Mahipal Ganji; Jaco van der Torre; Patrick Onck; Hendrik Dietz; Cees Dekker

doi:10.1038/s41467-018-03313-w

Nature Communications (Mar 2018)

DNA origami scaffold for studying intrinsically disordered proteins of the nuclear pore complex

Philip Ketterer,
Adithya N. Ananth,
Diederik S. Laman Trip,
Ankur Mishra,
Eva Bertosin,
Mahipal Ganji,
Jaco van der Torre,
Patrick Onck,
Hendrik Dietz,
Cees Dekker

Affiliations

Philip Ketterer: Physik Department and Institute for Advanced Study, Technische Universität München, Am Coulombwall 4a
Adithya N. Ananth: Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology
Diederik S. Laman Trip: Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology
Ankur Mishra: Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4
Eva Bertosin: Physik Department and Institute for Advanced Study, Technische Universität München, Am Coulombwall 4a
Mahipal Ganji: Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology
Jaco van der Torre: Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology
Patrick Onck: Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4
Hendrik Dietz: Physik Department and Institute for Advanced Study, Technische Universität München, Am Coulombwall 4a
Cees Dekker: Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology

DOI: https://doi.org/10.1038/s41467-018-03313-w
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 8

Abstract

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FG-Nups are disordered proteins in the nuclear pore complex (NPC) where they selectively control nuclear transport. Here authors build NPC-mimics based on DNA origami rings which attach a certain numbers of Nups to analyse those nanopores by cryoEM and conductance measurements.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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