Nature Communications (Jun 2021)
Engineering the protein dynamics of an ancestral luciferase
- Andrea Schenkmayerova,
- Gaspar P. Pinto,
- Martin Toul,
- Martin Marek,
- Lenka Hernychova,
- Joan Planas-Iglesias,
- Veronika Daniel Liskova,
- Daniel Pluskal,
- Michal Vasina,
- Stephane Emond,
- Mark Dörr,
- Radka Chaloupkova,
- David Bednar,
- Zbynek Prokop,
- Florian Hollfelder,
- Uwe T. Bornscheuer,
- Jiri Damborsky
Affiliations
- Andrea Schenkmayerova
- International Clinical Research Center, St. Anne’s University Hospital Brno
- Gaspar P. Pinto
- International Clinical Research Center, St. Anne’s University Hospital Brno
- Martin Toul
- International Clinical Research Center, St. Anne’s University Hospital Brno
- Martin Marek
- Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Faculty of Science, Masaryk University
- Lenka Hernychova
- Research Centre for Applied Molecular Oncology, Masaryk Memorial Cancer Institute
- Joan Planas-Iglesias
- International Clinical Research Center, St. Anne’s University Hospital Brno
- Veronika Daniel Liskova
- International Clinical Research Center, St. Anne’s University Hospital Brno
- Daniel Pluskal
- Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Faculty of Science, Masaryk University
- Michal Vasina
- International Clinical Research Center, St. Anne’s University Hospital Brno
- Stephane Emond
- Department of Biochemistry, University of Cambridge
- Mark Dörr
- Department of Biotechnology and Enzyme Catalysis, Institute of Biochemistry, University of Greifswald
- Radka Chaloupkova
- Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Faculty of Science, Masaryk University
- David Bednar
- International Clinical Research Center, St. Anne’s University Hospital Brno
- Zbynek Prokop
- International Clinical Research Center, St. Anne’s University Hospital Brno
- Florian Hollfelder
- Department of Biochemistry, University of Cambridge
- Uwe T. Bornscheuer
- Department of Biotechnology and Enzyme Catalysis, Institute of Biochemistry, University of Greifswald
- Jiri Damborsky
- International Clinical Research Center, St. Anne’s University Hospital Brno
- DOI
- https://doi.org/10.1038/s41467-021-23450-z
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 16
Abstract
Directed evolution commonly relies on point mutations but InDels frequently occur in evolution. Here the authors report a protein-engineering framework based on InDel mutagenesis and fragment transplantation resulting in greater catalysis and longer glow-type bioluminescence of the ancestral luciferase.
