Effect of Taka-diastase on the detection of vitamin B2 in beverages

Abstract

ObjectiveTo investigate and analyze the effect of Taka-diastase used for the detection of vitamin B2 （source of riboflavin-5'-sodium phosphate） on the results of the tested sample.MethodsAccording to the requirements of the national standard method GB 5009.85—2016， Method One， the content of vitamin B2 （source of riboflavin-5'-sodium phosphate） in the tested sample was determined to investigate the influence of Taka-diastase and its operational conditions on the quantification results.ResultsTaka-diastase from different sources had a major effect on the vitamin B2 （source of riboflavin-5'-sodium phosphate） measurement results， and the maximum difference between the measured and theoretical values was more than 10 fold. The results indicated that an enzymatic hydrolysis time of 12 h， and pH 6.0—6.5 were optimal.ConclusionWhen the national standard method was used to detect vitamin B2 （source of riboflavin-5'-sodium phosphate） in beverages， the selection of Taka-diastase had a significant effect on the detection results.

Keywords

• vitamin b2
• riboflavin-5'-sodium phosphate
• food additives
• taka-diastase
• hplc