Effect of the alcohol extract of the Lipidium sativum and Bacillus cereus bacteria in the growth of fungi causing sesame root rot

Abstract

Abstract The effect of the concentrations 0,1, 2, 3 and 4 mg/ml of alcoholic extracts of cress seeds) Lipidium sativum (on the growth and dry weight of root–rot fungi of sesame plants, Pythium aphanidermatum, Fusarium solani and Macrophomina phaseolina indicated high significant inhibitory affect as compared to the control. M.phaseolina was mostly inhibited than other fungi when 4mg/ml w, 86.66 and 78.26% respectively. Antagonistic test of the bacterial biocontrol agent Bacillus cereus showed high inhibiting effect on all tested pathogens with the maximum inhibition 80.8% on M. phaseolina. Culture filterate of B. cereus also showed a highly inhibiting efficiency to the growth and dry wieght of the biomass of all pathogenic fungi with the increase of concentrations 10%, 20%, 30% and 40% (v l v), With the 40% was mostly effective on M. phaseolina by the ratio of 72.22% and 83.90% respectively. The best inhibition was achieved with the use of combination of 4 mg/ml of alcoholic extract of Cress seeds and 40% of culture filterate of Bacillus cereus. It showed synergistic inhibitory effect on all pathogenic fungi used, that exceeded the effect of each of the plant extract or culture filterate of the bacteria separtely.

Keywords

• alcoholic extracts
• lipidium sativum
• sesame plants