A comparative cytological study of silver and molybdenum oxide nanostructures against breast cancer cells

Abstract

Cancer is a denying disease, and among a number of cancers types, breast cancer is a recurrent in females, which affect word widely. For these reasons, the work presented here to show the comparative cytological responses of breast (MCF-7) cancer cells with silver and molybdenum oxide nanostructures. The nanostructures were produced via chemical process and characterized. The crystallinities, their size and phases were determined with X-ray diffraction pattern (XRD), while the structural details were scrutinized via scanning electron microscopy (SEM) and transmission electron microscopy (TEM) correspondingly. The nanorods (NRs) and nanoparticles (NPs) were exhibit and confirmed individual diameter of ∼50–60 nm and ∼15 nm separately. A cytotoxicity’s studies of MCF-7 cells were sway and reliant to their doses (1 to 100 μL/mL) with α-MoO3NRs and AgNPs established via 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) and Neutral Red Uptake (NRU) assays. The reactive oxygen species (ROS) were also upsurges slowly from 105, 124, 132, 148 and 108, 130,135, 165 with dissimilar concentrations of α-MoO3NRs and AgNPs (25, 50 and 100 μL/mL) with control reciprocally. Also gene expression (mRNA extraction) study with apoptotic markers in presence of α-MoO3NRs and AgNPs were up-regulated and shows apoptosis in cells. The study provides a possible comparative results related to the cytotoxicity’s in breast (MCF-7) cancer cell with AgNPs are high as compared to α-MoO3NRs also authenticated via gene expression study.

Keywords

• Molybdenum oxide NRs
• Silver NPs
• Cytotoxicity
• MCF-7 cells
• ROS
• RT-PCR