Retinal apoptosis and ultrastructural features of defocus-induced myopia in guinea pigs

Abstract

AIM: To observe retinal ultrastructural features and retinal cell apoptosis of defocus induced myopia in guinea pigs. METHODS: Thirty guinea pigs(3-wk old)were received -10.00D rigid contact lenses(RGP)on the right eye and without wearing glasses on the left eye. Diopter change with cycloplegic retinoscopy, ultrastructural features of the retina with transmission electron microscopy were observed, the apoptosis of retinal cell by TUNEL immunofluorescence staining after 3wk were detected.RESULTS: Eye diopter difference was 3.28±0.21D in the right eye and 1.55±0.23D in the left eye before and after induction. There was significantly statistical difference(PPCONCLUSION: Accompanied by abnormal apoptosis of retinal cells during the formation of defocus-induced myopia in guinea pigs.

Keywords

• defocus-induced
• guinea pigs
• myopia
• retinal ultrastructural features
• apoptosis