Journal of Food Protection (Oct 2023)
Persistence of Salmonella enterica subsp. enterica ser. Javiana, Listeria monocytogenes, and Listeria innocua in Hydroponic Nutrient Solution
Abstract
This study aimed to determine the persistence of Salmonella Javiana, Listeria monocytogenes, and Listeria innocua in nonsterile, hydroponic nutrient solution (NS) at 15, 25, 30, and 37°C over a 21-day period to mimic time from seedling to mature lettuce. Bacteria were inoculated in modified Hoagland's NS at 106 CFU/mL and maintained at 15, 25, 30, and 37°C. Samples were collected at various time points, and bacteria were quantified. A mixed model was used to determine the effect of bacteria type, time (day), and temperature on bacteria concentration (log CFU/mL). The least-squares means were calculated to compare the mean log CFU/mL, and the mean values were compared with Tukey-Kramer honest significant difference test with a significance level of P = 0.05. Statistical analysis indicated that a 3-way interaction effect between temperature, time, and bacteria type had a significant impact on bacterial persistence in NS (P < 0.0001). At all temperatures, S. Javiana persisted in NS throughout the 21-day study period, compared to L. innocua and L. monocytogenes where persistence was limited to between 1 and 14 days. Similarly, decimal reduction values (D-values) of S. Javiana indicated longer persistence in NS than L. innocua and L. monocytogenes at most temperatures. For instance, at 15°C and 25°C, D-values for S. Javiana were estimated at 82 and 26 d, respectively, compared to D-values of 3.6 and ∼3 d for L. monocytogenes. Data indicate that the temperature of NS has a differential effect on the persistence of S. Javiana and Listeria spp. This study furthers the understanding of potential food safety risks associated with hydroponic systems and will contribute to the refinement of further studies to aid in the development of operation-specific risk profiles.
