First evidence of antimicrobial activity of neurotoxin 2 from Anemonia sulcata (Cnidaria)

MR Trapani; MG Parisi; M Toubiana; L Coquet; T Jouenne; P Roch; M Cammarata

Invertebrate Survival Journal (Jun 2014)

First evidence of antimicrobial activity of neurotoxin 2 from Anemonia sulcata (Cnidaria)

MR Trapani,
MG Parisi,
M Toubiana,
L Coquet,
T Jouenne,
P Roch,
M Cammarata

Affiliations

MR Trapani: Marine Immunobiology Laboratory, Department of Biological, Chemical, Pharmaceutical Science and Technology, University of Palermo, Palermo, Italy; Ecologie des Systèmes Marins Côtiers, CNRS-Université Montpellier 2, place E. Bataillon, Montpellier, France
MG Parisi: Marine Immunobiology Laboratory, Department of Biological, Chemical, Pharmaceutical Science and Technology, University of Palermo, Palermo, Italy
M Toubiana: Ecologie des Systèmes Marins Côtiers, CNRS-Université Montpellier 2, place E. Bataillon, Montpellier, France
L Coquet: Plateforme de Protéomique PISSARO, CNRS-Université de Rouen, Place E. Blondel, Mont-Saint-Aignan, France
T Jouenne: Plateforme de Protéomique PISSARO, CNRS-Université de Rouen, Place E. Blondel, Mont-Saint-Aignan, France
P Roch: Ecologie des Systèmes Marins Côtiers, CNRS-Université Montpellier 2, place E. Bataillon, Montpellier, France
M Cammarata: Marine Immunobiology Laboratory, Department of Biological, Chemical, Pharmaceutical Science and Technology, University of Palermo, Palermo, Italy

Journal volume & issue: Vol. 11
pp. 182 – 191

Abstract

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We investigated the antibacterial activity of Anemonia sulcata (Cnidaria, Anthozoa) tentacle and body acidic extracts. Biochemical purification consisted of first step on solid phase Sep-Pak C8 column followed by several HPLC runs on C18 column using different conditions. Anti-Micrococcus lysodeikticus activity has been detected in 40 % acetonitrile fractions. The resulting purified molecule from tentacles had a molecular mass determined by MALDI-TOF mass spectrum of 4946,299 Da and has been completely sequenced. Its aa sequence revealed identity with the Neurotoxin 2 (ATX-II), a Na+ channel blocking toxins. Consequently, ATX-II appeared to display a dual role as toxin and as antibacterial.

antimicrobial peptide; Anemonia sulcata; ATX II; neurotoxin; Micrococcus lysodeikticus

Published in Invertebrate Survival Journal

ISSN: 1824-307X (Print)
Publisher: ISJ
Country of publisher: Italy
LCC subjects: Science: Biology (General)
Website: http://www.isj.unimo.it/

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