Frontiers in Bioengineering and Biotechnology (Nov 2021)

Use of Electrospun Phenylalanine/Poly-ε-Caprolactone Chiral Hybrid Scaffolds to Promote Endothelial Remodeling

  • Benlin Sun,
  • Lei Hou,
  • Binbin Sun,
  • Binbin Sun,
  • Yu Han,
  • Yu Han,
  • Yunqing Zou,
  • Juexin Huang,
  • Yanan Zhang,
  • Chuanliang Feng,
  • Xiaoqiu Dou,
  • Feng Xu,
  • Feng Xu

DOI
https://doi.org/10.3389/fbioe.2021.773635
Journal volume & issue
Vol. 9

Abstract

Read online

The fabrication of tissue-engineered vascular grafts to replace damaged vessels is a promising therapy for cardiovascular diseases. Endothelial remodeling in the lumen of TEVGs is critical for successful revascularization. However, the construction of well-functioning TEVGs remains a fundamental challenge. Herein, chiral hybrid scaffolds were prepared by electrospinning using D/L-phenylalanine based gelators [D(L)PHEG] and poly-ε-caprolactone (PCL). The chirality of scaffolds significantly affected the endothelial remodeling progress of TEVGs. Compared with L-phenylalanine based gelators/poly-ε-caprolactone (L/PCL) and PCL, D-phenylalanine based gelators/poly-ε-caprolactone (D/PCL) scaffolds enhanced cell adhesion, and proliferation and upregulated the expression of fibronectin-1, and vinculin. These results suggests that chiral hybrid scaffolds can promote endothelial remodeling of TEVGs by upregulating adhesion-associated protein levels. This study offers an innovative strategy for endothelial remodeling of TEVGs by fabricating chiral hybrid scaffolds, and provides new insight for the treatment of cardiovascular diseases.

Keywords