Intervention study of Rosa roxbunghii polysaccharide extracts on Type Ⅱ diabetes in mice

Abstract

To investigate the synergistic effect of Rosa roxbunghii Tratt. polysaccharide（RRTP）and Rosa roxbunghii Tratt. insoluble dietary fiber（RTIDF）on hypoglycemic function. In this study, RRTP and RTIDF were extracted, isolated and purified, the antioxidant and hypoglycemic activities of polysaccharides were measured by in vitro experiment and the relationship between hypoglycemic function and intestinal microbial community structure in mice was analyzed in vivo intervention experiment of Type Ⅱ diabetes in mice. The results were as follows: (1) RRTP had a good free radical scavenging ability in vitro, and could significantly inhibit α-glucosidase and α-amylase activities with IC50 values of 0.293 and 4.251 mg·mL-1, respectively. RTIDF only showed certain inhibitory activity on α-amylase activity. (2) After the intervention of the extracts in the model mice, the tendency of the obese mice to continue to lose weight was reversed. Compared with CK group (physiological saline), the blood glucose level of RTIDF and RRTP+RTIDF mice were significantly down-regulated, and the activity of CAT enzyme in serum was significantly enhanced. RRTP+RTIDF group was superior to RTIDF group. (3) The extract intervention could reduce the inflammatory factors in the liver, relieve the degree of cell swelling, increase the number of absorbing cells in the cecum, and restore the intestinal wall mucosal layer. (4) Further analysis of intestinal microbial community showed that RTIDF+RRTP could reduce the proportion of Bacteroidetes and Firmicutes, increase the abundance of beneficial bacteria such as Acetobacter, but RTIDF had more significant regulation effect on the population. Therefore, based on in vitro hypoglycemic simulation and in vivo intervention results, RRTP and RTIDF have a certain synergistic effect on glucose intervention in diabetic mice, it may be used together as an intervention to improve Type Ⅱ diabetes.

Keywords

• rosa roxbunghii tratt. polysaccharide
• dietary cellulose
• isolation and purification
• type ⅱ diabetes
• blood glucose intervention