Food and Agricultural Immunology (Jan 2018)

Immunochromatographic paper sensor for ultrasensitive colorimetric detection of cadmium

  • Shanshan Song,
  • Shuzhen Zou,
  • Jianping Zhu,
  • Liqiang Liu,
  • Hua Kuang

DOI
https://doi.org/10.1080/09540105.2017.1354358
Journal volume & issue
Vol. 29, no. 1
pp. 3 – 13

Abstract

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A novel and highly sensitive immunochromatographic strip based on a monoclonal antibody (3A9) was developed for the detection of cadmium in tap water. The 50% inhibition concentration of the antibody, which showed no cross-reactivity with other heavy metal ions, was 0.45 ng/mL and it recognized Cd(II)–1-(4-isothiocyanobenzyl) ethylenediamine-N,N,N′,N′-tetraacetic acid (EDTA) (Cd(II)–ITCBE) and not metal-free ITCBE and EDTA. The cutoff value for semi-quantitative detection of the strip was 5 ng/mL and the lower limit of detection for quantitative detection was 0.2 ng/mL using a scanning reader. The percent recovery ranged from 107.6% to 132% in tap water samples.

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